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rabbit polyclonal anti human pot1  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal anti human pot1
    Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of <t>POT1</t> (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.
    Rabbit Polyclonal Anti Human Pot1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human pot1/product/Novus Biologicals
    Average 94 stars, based on 21 article reviews
    rabbit polyclonal anti human pot1 - by Bioz Stars, 2026-05
    94/100 stars

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    1) Product Images from "Telomere protein RAP1 levels are affected by cellular aging and oxidative stress."

    Article Title: Telomere protein RAP1 levels are affected by cellular aging and oxidative stress.

    Journal: Biomedical reports

    doi: 10.3892/br.2016.707

    Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of POT1 (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.
    Figure Legend Snippet: Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of POT1 (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.

    Techniques Used: Control, Quantitation Assay



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    rabbit polyclonal anti human pot1  (Novus Biologicals)
    94
    Novus Biologicals rabbit polyclonal anti human pot1
    Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of <t>POT1</t> (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.
    Rabbit Polyclonal Anti Human Pot1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human pot1/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal anti human pot1 - by Bioz Stars, 2026-05
    94/100 stars
    		  Buy from Supplier

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    Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of POT1 (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.

    Journal: Biomedical reports

    Article Title: Telomere protein RAP1 levels are affected by cellular aging and oxidative stress.

    doi: 10.3892/br.2016.707

    Figure Lengend Snippet: Figure 2. Shelterin levels in young and old HDFn cells. (A) The levels of TRF2 (upper panel) and RAP1 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL28. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). The secondary antibodies used were for detection using the Odyssey CLx imager quantitation program (LI‑COR Biosciences). Asterisks indicate the appropriate bands for the proteins of interest. Measurements were done using the Odyssey CLx imager quantitation program. Values for TRF2 and RAP1 were normalized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. TRF2 levels in the PDL28 samples were 36% of the PDL6 levels. RAP1 at PDL28 was 75% of the PDL6 levels. (B) The levels of POT1 (upper panel) and TIN2 (middle panel) were compared in young HDFn cells at PDL6 and old HDFn cells at PDL32. Various amounts of whole cell extracts were resolved by SDS‑PAGE, and the blotted proteins were detected with specific antibodies. Actin was used as a loading control (lower panel). Asterisks indicate the appropriate bands for the proteins of interest. Measurements for the appropriate bands on the film were done using NIH ImageJ. Values for POT1 and TIN2 were nor malized by those for actin. The values calculated for each lane of one protein were averaged for each PDL. Levels of both POT1 and TIN2 in the older cells were approximately the same as those in the younger cells. For both A and B, all portions of the figure were from the same blot with irrelevant information deleted for visual clarity.

    Article Snippet: The primary antibodies used in the study were obtained from the following vendors: mouse monoclonal anti-human TRF2 (cat. no. 05-521) from Calbiochem (Billerica, MA, USA) used at 1:1,000 dilution; rabbit polyclonal anti-human RAP1 (cat. no. A300-306A) from Bethyl Laboratories (Montgomery, TX, USA) used at 1:2,000 dilution; rabbit polyclonal anti-human TIN2 (cat. no. ab82998) from Abcam Inc. (Cambridge, MA, USA) used at 1:1,000 dilution; rabbit polyclonal anti-human POT1 (cat. no. NB500-176) from Novus Biologicals (Littleton, CO, USA) used at 1:1,000 dilution; and goat polyclonal anti-human actin (cat. no. sc-1616) from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) used at 1:2,000 dilution.

    Techniques: Control, Quantitation Assay